MITIGATIVE EFFECTS OF NIGELLA SATIVA ON THE HISTOLOGY OF KIDNEYS AGAINST ASPIRIN-INDUCED TOXICITY

Authors

  • Sania Asif King Edward Medical University, Lahore
  • Rabia Sajjad Toor University of Health Sciences, Lahore
  • Sitwat Amna
  • Areeba Haider
  • Sarah Khan

Abstract

Background: Aspirin is one of the popular, economical, easily accessible and commonly used drugs all over the world. Injudicious use of this over-the-counter available drug is a common cause of nephrotoxicity. The aim of the present study is to assess the protective effects of Nigella Sativa (NS) on the histology of kidneys against aspirin-induced toxicity.  It was an experimental study that included comparative analysis of control and experimental groups, conducted in the department of Anatomy, University of Health Sciences, Lahore, from October 2016 to December 2016. Methods: The study included thirty-two female albino rats which were equally distributed into 4 groups. Group A was run as control and given single oral dose of 1% methyl-cellulose (10mg/100gm body weight of rat). Group B and C were treated with a single oral dose of aspirin (1000mg/kg body weight) dissolved in 1% methyl-cellulose (10mg/100gm body weight). Group C animals were left untreated for 7 days. Group D was pre-treated on day 1 with oral dose of Nigella Sativa (NS) extract (250mg/kg body weight) followed one hour later by a single oral dose of aspirin (1000mg/kg body weight), subsequently NS extract was administered till day 7. Rats of group B were euthanized and dissected on 2nd day of experiment while those of groups A, C and D on 8th day. Kidneys were dissected out, weighed and fixed in 10% formalin. 5µm thick sections were yielded after tissue processing and stained with haematoxylin, eosin (H&E staining) and periodic acid Schiff’s reagent (PAS staining). Histological parameters of distal convoluted tubules (DCT) were observed. Results: All histological parameters were normal in group A. Group B showed marked increase in epithelial necrosis, intraluminal protein casts and broken basement membranes of distal convoluted tubules. Group C showed no self-recovery. Statistically significant improvement was observed in the histology of distal convoluted tubules with treatment of Nigella Sativa extract in aspirin-ingested rats in group D. Conclusion: Nigella Sativa extract has shown protective effects on kidneys against aspirin-induced damage as shown by improvement in the histological parameters of distal convoluted tubules. Keywords: Kidneys; Nigella Sativa; Aspirin; Distal convoluted tubules; Methyl-cellulose

Author Biographies

Sania Asif, King Edward Medical University, Lahore

Assitant Professor, Anatomy department, King Edward Medical University, Lahore

Rabia Sajjad Toor, University of Health Sciences, Lahore

Assitant Professor, Anatomy department, Sahara Medical College, Narowal

Sitwat Amna

Assitant Professor, Anatomy department, Gujranwala Medical College

Areeba Haider

Assitant Professor, Anatomy department, King Edward Medical University, Lahore

Sarah Khan

Assitant Professor, Anatomy department, King Edward Medical University, Lahore

References

Fuster V, Sweemy JM. Apirin, a historical and contemporary therapeutic overview. Circulation, 2011; 123: 768-778.

Zhou Y, Boudreau DM, Freedman AN. Trends in the use of aspirin and non-steroidal anti-inflammatory drugs in general U.S. population. Pharmacol Drug safety, 2013; 23(1): 43-50. doi: 10.1002/pds.3463.

Milland MA, Hernandez-vila EA. What do the guidelines really say about aspirin? Tex Heart Inst J, 2018; 45(4): 228-30. doi: 10.14503/THIJ-18-6673.

Ittaman SV, VanWormer JJ, Rezkalla SH. The role of aspirin in the prevention of cardiovascular disease. Clin Med Res, 2014; 12(3-4): 147-54. doi: 10.3121/cmr.2013.1197.

Chubak J, Whitlock EP, Williams SB, Kamineni A, Burda BV, Buist DS, Anderson ML. Aspirin for the prevention of cancer incidence and mortality: systemic evidence reviews for the U.S. Preventive Services Task Force. Ann Intern Med, 2016; 164(12): 814-25.

Vanwormer JJ, Greenlee RT, McBride PE, Peppard PE, Malecki KC, Che J, Nieto FJ. Aspirin for primary prevention of CVD: are the right people using it? J Fam Pract, 2012; 61:525533.

Bozimowski G. A review of non-steroidal anti-inflammatory drugs. AANA J, 2015; 83(6): 425-33.

Shahbazi F, Khavidaki SD, Khalili H, Pezeshki ML. Potential

renoprotective effects of silymarin against nephrotoxic drugs: a review of literature. J Pharm Pharmaceut Sci, 2012; 15(1): 112-23.

Bouzema H, Dhibi S, Samout N, Rjeibin I, Talamin H, Elfeki A, Hfaiedh N. The protective effect of citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin. J Biomed Pharmacother, 2016; 83:1327-34. doi: 10.1016/jbioph.2016.08.037.

Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed, 2013; 3(5): 337-52. doi: 10.1016/S2221-1691(13)60075-1.

Amin B, Hosseinzadeh H. Black cumin (nigella sativa) and its active constituent, thymoquinone: an overview on the analgesic and anti-inflammatory effects. Planta Med, 2016; 82(01/02): 8-16. doi: 10.1055/s-0035-1557838.

Tavakkoli A, Mahdian V, Razavi BM, Hosseinzadeh H. Review on clinical trials of black seed (Nigella sativa) and its active constituent, thymoquinone. J Pharmacopuncture, 2017; 20(3): 179-93. doi: 10.3831/KPI.2017.20.021.

Heiss AG, Stika HP, DeZorzi N, Jursa M. Nigella in the mirror of time a brief attempt to draw a genus’ ethnohistorical portrait. Offa, 2012; 69/70:147-69.

Beheshti F, Norouzi F, Abareshi A, Khazaei M, Alikhani V, Moussavi S, Biglari G, Soukhtanloo M, Hosseini M. Nigella Sativa prevented liver and renal tissue damage in lipopolysaccharide-treated rats. Saudi J Kidney Dis Transpl, 2018; 29: 554-66.

Fuster V, Sweeny JM. A historical and contemporary therapeutic overview. American heart association, 2015; 123: 768-778.

Gaziano JM, Brotons C, Coppolechia R. Use of aspirin to reduce the risk of initial vascular events in patients at moderate risk of cardiovascular disease (ARRIVE): a randomized double-blind, placebo-controlled trial. Lancet, 2018; 392 (10152): 1036-46. doi: 10.1016/S0140-6736(18)31924-X.

Chubak J, Whitlock EP, Williams SB. Aspirin for the prevention of cancer incidence and mortality: systemic evidence reviews of the U.S. Preventive Services Task Force. Ann Intern Med, 2016; 164 (12): 814-25. doi: 10.107326/M15-2117.

Khalil MAM, Khalil MSUD, Khanes SSA, Alam S, Daiwajna RG, Rajput AS, Alhaj MM, et al. Pros and cons of aspirin prophylaxis for prevention of cardiovascular events in kidney transplant and review of evidence. Ad Prev Med, 2019: e6139253. doi: 10.1155/2019/6139253.

Boon AC, Lam AK, Benzie IF, Coomber J, Fassett RG, Blume AC. Endogenous elevated bilirubin modulates kidney function & protection from circulating oxidative stress in a rat model of adenine induced kidney failure. Scientific reports, 2015; 5: 1-16.

Jain N, Shrivastava R, Raghuwanshi AK, Shrivastava VK. Aspirin induced changes in serum ACP, ALP, GOT, GPT, BILIRUBIN and CREATININE in correlation with histopathological changes in liver and kidney of female albino rat. International Journal of Applied Pharmaceutics, 2012; 4(3): 9-11.

Okada S, Morimoto T, Ogawa H, Mio S, Soejima H, Nakayama M, et al. Is long term low dose aspirin therapy associated with renal dysfunction in patients with type 2 diabetes? JPAP2 cohort study. J PLoS One, 2016; 11(1): e0147635. doi: 10.1371/Journal.pone.0147635.

Hosseinian S, Rad KA, Hadizadeh MAR, Roshan NM, Havakhah S, SHafiee S. The protective effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats. Avicenna J Phytomed, 2016; 6(1):44-54.

Havakhah S, Sadeghnia HR, Hajzadeh MR, Mohammadian N, Safiee S, Hosseinzadeh H, Mohareri N, et al. Effect of nigella sativa on ischemia-reperfusion induced rat kidney damage. Iranian J Basic Med Sci, 2014; 17: 986-92.

Published

2021-02-20

Issue

Vol. 33 No. 1 (2021): Journal of Ayub Medical College, Abbottabad

Section

ORIGINAL ARTICLE

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