PLASTINATION OF WHOLE BRAIN SPECIMEN AND BRAIN SLICES

Authors

  • ARBAB ABDUL WADOOD
  • ABDUL JABBAR
  • NIRMAL DAS

Abstract

Background: The human dead body specimens are plastinated for teaching purposes in medical institutions, using
silicone. The silicone impregnated whole brain specimens and brain slices do not give satisfactory results. Methods:
In the present study the brain specimens were plastinated with another polymer known as Polyester-Copolymer. The
brain specimens were first preserved and then fixed with 5% formalin. The specimens were then dehydrated and
degreased in a volatile solvent acetone. The specimens were placed in Polyester- Copolymer solution which penetrated
the brain tissue both intracellulary and intercelluraly. The specimens were then cured by gas method. Results: The
whole brain specimens and brain slices plastinated with Polyester-Copolymer were dry, odorless, handy and durable.
It also gives a clear visual contrast between grey and white matter in brain slices whereas the brain specimens
plastinated with silicone are flexible and sticky. There was no color contrast between grey and white matter.
Conclusion: The polyester impregnated brain specimens and slices are non-toxic and ideal for teaching purposes and
examinations. They require minimal aftercare. The whole organ serial sections of plastinated brain specimens will
help 3- dimensional study of the normal brain and will improve the assessment of brain pathology.

References

Hagens G. Plastination technique. Heidel. Plast. Folder. l986;

l-7.

Brickley H C. Walker A N and Jackson R L and Donnars R S.

The preservation of pathology specimens by silicone

plastination An innovative adjunct to pathology education.

Am.J. Clin Pathol. 1987;88(2):220-23.

Hagens G. Impregnation of soft biological specimens with

thermosetting resins and elastomers. Anat. Rec. 1979; 194;

-255

Klanes T and Wilhelm K. The current potential of plastination

Anat. Embr. 1987; 175:411-21.

Hagens G, Kriz. Plast. Folder. 1985;5-17.

Hagens and Tiedemann Plastination. Anat. Embr. 1984;210-

Schwab K H and Hagens G. Freeze substitution of

macroscopic specimens for plastination. Abstract. Sixth

European Anatomical Congress. Acta. Anat. 1981, 111(12)

Kieneke E W. Uhlmann K. Easy method of producing

biological specimens by molecular substitution. XII.

Int Anat Congr London. Abstr. 1985; 354.

Sittel C. Eckel H E and Sprinzl G M. Section plastination of

larynx for histology of whole organ sections. HNO. 1996;

(7)370-5

Stoka K and Schit G. Utilization of the postmortem

examination with emphasis on audiovisual aids. Arch Pathol.

Lab Med. 1987;11 U9):883-84.

Hawley DA. Marlin DC, Cook DC, Becsay D, Clark M A,

Pless J I; and Stanish S M. Specimens for teaching forensic

pathology, odontology and anthropology. Am.J. ForensicMed. Pathol 1991; 12(2): 170-174.

Deegner P and Berndta W. Process of preserving animal

object. U.S. Pat.l914; l, 163,645.

Acheson E D Formaldehyde in British chemical industry.

Lancet. 1981; 1:611-616.

Daschner F. Formaldehyde. Klinikartz. 1983; 12(1): 9-10.

Pabst R Wetche Gefahren bestehen beim umgang mit.

Formaldehyde. Anat, Anz. 1985; 161:154.

Bickley H C. Plastination, a new technique for anatomic

pathology and forensic science. Pathol. Update Series.

;2(16);2-8.

Bickly H C, Hagens G and Townsend F M. An improved

method for preservation of teaching specimens. Arch. Pathol.

Lab. Med. 1981;105: 674-676.

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How to Cite

WADOOD, A. A., JABBAR, A., & DAS, N. (2001). PLASTINATION OF WHOLE BRAIN SPECIMEN AND BRAIN SLICES. Journal of Ayub Medical College Abbottabad, 13(1), 11–13. Retrieved from https://www.jamc.ayubmed.edu.pk/jamc/index.php/jamc/article/view/4834